Robert Koch's Postulates About Tuberculosis🔬🔖
Postulate:
1. The microorganism must be
present in every case of the
disease but absent from
healthy organisms.
Experimentation:
Koch developed a staining technique to examine human tissue. Mycobacterium tuberculosis could be identified in
diseased tissue.
Postulate:
2. The suspected microorganisms
must be isolated and grown in a
pure culture.
Experimentation:
Koch grew M. tuberculosis in pure
culture on coagulated blood serum.
Postulate:
3. The same disease must result
when the isolated microorganism
is inoculated into a healthy host.
Experimentation:
Koch injected cells from the pure
culture of M. tuberculosis into guinea
pigs. The guinea pigs subsequently
died of tuberculosis.
Postulate:
4. The same microorganisms must
be isolated again from the
diseased host.
Experimentation:
Koch isolated M. tuberculosis in pure culture on coagulated blood serum from the dead guinea pigs.
Source: Prescott’s Microbiology📄🔖
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